| pubmed-article:1894938 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1894938 | lifeskim:mentions | umls-concept:C0043474 | lld:lifeskim |
| pubmed-article:1894938 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1894938 | lifeskim:mentions | umls-concept:C0028630 | lld:lifeskim |
| pubmed-article:1894938 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:1894938 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1894938 | pubmed:dateCreated | 1991-10-22 | lld:pubmed |
| pubmed-article:1894938 | pubmed:abstractText | An assay to quantify the phosphorylation products of zidovudine (AZT) in peripheral blood mononuclear cells (PBMC) was developed. Extracts of PBMC were separated by high-performance liquid chromatography. Eluted AZT mono- (MP), di- (DP), and triphosphate (TP) were collected in separate portions. Treatment with alkaline phosphatase yielded equimolar amounts of AZT, which after solid-phase enrichment were assayed by radioimmunoassay. Detection limit was 0.1 pmol/10(6) PBMC for each nucleotide. Recoveries of 102%-118% were observed. AZT nucleotides were measured in samples from three patients receiving 250 mg of AZT every 12 h. Intracellular concentrations of AZT-MP after 1-2 h ranged from 0.9 to 1.4 pmol/10(6) PBMC and then declined to 0.3-1.1 pmol/10(6) PBMC after 4 h. AZT-DP and AZT-TP reached concentrations of 0.3-0.5 pmol/10(6) PBMC after 1-2 h and could not be detected after 4 h in any of the three patients. Duplicate determinations deviated by less than 20%. | lld:pubmed |
| pubmed-article:1894938 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1894938 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1894938 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:1894938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1894938 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1894938 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:1894938 | pubmed:issn | 0022-1899 | lld:pubmed |
| pubmed-article:1894938 | pubmed:author | pubmed-author:VogtMM | lld:pubmed |
| pubmed-article:1894938 | pubmed:author | pubmed-author:LüthyRR | lld:pubmed |
| pubmed-article:1894938 | pubmed:author | pubmed-author:KusterHH | lld:pubmed |
| pubmed-article:1894938 | pubmed:author | pubmed-author:JoosBB | lld:pubmed |
| pubmed-article:1894938 | pubmed:author | pubmed-author:NadaiVV | lld:pubmed |
| pubmed-article:1894938 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1894938 | pubmed:volume | 164 | lld:pubmed |
| pubmed-article:1894938 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1894938 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1894938 | pubmed:pagination | 773-6 | lld:pubmed |
| pubmed-article:1894938 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
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| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:meshHeading | pubmed-meshheading:1894938-... | lld:pubmed |
| pubmed-article:1894938 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1894938 | pubmed:articleTitle | A method for the quantification of intracellular zidovudine nucleotides. | lld:pubmed |
| pubmed-article:1894938 | pubmed:affiliation | Department of Medicine, University Hospital, Zurich, Switzerland. | lld:pubmed |
| pubmed-article:1894938 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1894938 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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