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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1991-10-22
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pubmed:abstractText |
An assay to quantify the phosphorylation products of zidovudine (AZT) in peripheral blood mononuclear cells (PBMC) was developed. Extracts of PBMC were separated by high-performance liquid chromatography. Eluted AZT mono- (MP), di- (DP), and triphosphate (TP) were collected in separate portions. Treatment with alkaline phosphatase yielded equimolar amounts of AZT, which after solid-phase enrichment were assayed by radioimmunoassay. Detection limit was 0.1 pmol/10(6) PBMC for each nucleotide. Recoveries of 102%-118% were observed. AZT nucleotides were measured in samples from three patients receiving 250 mg of AZT every 12 h. Intracellular concentrations of AZT-MP after 1-2 h ranged from 0.9 to 1.4 pmol/10(6) PBMC and then declined to 0.3-1.1 pmol/10(6) PBMC after 4 h. AZT-DP and AZT-TP reached concentrations of 0.3-0.5 pmol/10(6) PBMC after 1-2 h and could not be detected after 4 h in any of the three patients. Duplicate determinations deviated by less than 20%.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
773-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1894938-Alkaline Phosphatase,
pubmed-meshheading:1894938-Cells, Cultured,
pubmed-meshheading:1894938-Centrifugation, Density Gradient,
pubmed-meshheading:1894938-Chromatography, High Pressure Liquid,
pubmed-meshheading:1894938-Dideoxynucleotides,
pubmed-meshheading:1894938-Humans,
pubmed-meshheading:1894938-Leukocytes, Mononuclear,
pubmed-meshheading:1894938-Phosphorylation,
pubmed-meshheading:1894938-Radioimmunoassay,
pubmed-meshheading:1894938-Thymine Nucleotides,
pubmed-meshheading:1894938-Zidovudine
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pubmed:year |
1991
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pubmed:articleTitle |
A method for the quantification of intracellular zidovudine nucleotides.
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pubmed:affiliation |
Department of Medicine, University Hospital, Zurich, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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