Linked Life Data

18948093

Source:http://linkedlifedata.com/resource/pubmed/id/18948093

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-12-1
pubmed:abstractText
Skeletal muscles of vertebrates are typically composed of slow- and fast-twitch fibers that differ in their morphology, gene expression profiles, contraction speeds, metabolic properties and patterns of innervation. During myogenesis, how muscle precursors are induced to mature into distinct slow- or fast-twitch fiber-types is inadequately understood. We have previously shown that within the somites of the zebrafish embryo, the activity of the zinc finger and SET domain-containing transcriptional regulator Blimp1 is essential for the specification of slow muscle fibers. Here, we have investigated the mechanism by which Blimp1 programs myoblasts to adopt the slow-twitch fiber fate. In slow myoblasts, expression of the Blimp1 protein is transient, and precedes the expression of slow muscle-specific differentiation genes. We demonstrate that the competence of somitic myoblasts to commit to the slow lineage in response to Blimp1 changes as a function of developmental time. Furthermore, we provide evidence that mammalian Blimp1 can recapitulate the slow myogenic program in zebrafish, suggesting that zebrafish Blimp1 can recognize the same consensus DNA sequence that is bound by the mammalian protein. Finally, we show that zebrafish Blimp1 can repress the expression of fast muscle-specific myosin light chain, mylz2, through direct binding near the promoter of this gene, indicating that an important function of the transcriptional activity of Blimp1 in slow muscle development is the suppression of fast muscle-specific gene expression. Taken together, these findings provide new insights into the molecular basis of vertebrate muscle fiber-type specification, and underscore Blimp1 as the central determinant of this process.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1095-564X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
324
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
226-35
pubmed:dateRevised
2009-5-19
pubmed:meshHeading
pubmed-meshheading:18948093-Animals, pubmed-meshheading:18948093-Animals, Genetically Modified, pubmed-meshheading:18948093-Binding Sites, pubmed-meshheading:18948093-Cardiac Myosins, pubmed-meshheading:18948093-Cell Differentiation, pubmed-meshheading:18948093-Chromatin Immunoprecipitation, pubmed-meshheading:18948093-DNA-Binding Proteins, pubmed-meshheading:18948093-Embryo, Nonmammalian, pubmed-meshheading:18948093-Gene Expression Regulation, Developmental, pubmed-meshheading:18948093-Mice, pubmed-meshheading:18948093-Muscle Development, pubmed-meshheading:18948093-Muscle Fibers, Slow-Twitch, pubmed-meshheading:18948093-Myoblasts, Skeletal, pubmed-meshheading:18948093-Myosin Light Chains, pubmed-meshheading:18948093-Nuclear Proteins, pubmed-meshheading:18948093-Promoter Regions, Genetic, pubmed-meshheading:18948093-Transcription, Genetic, pubmed-meshheading:18948093-Zebrafish, pubmed-meshheading:18948093-Zebrafish Proteins
pubmed:year
2008
pubmed:articleTitle
Specification of vertebrate slow-twitch muscle fiber fate by the transcriptional regulator Blimp1.
pubmed:affiliation
Institute of Molecular and Cell Biology, Cancer and Developmental Cell Biology Division, Proteos, 61 Biopolis Drive, Singapore 138673, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't