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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-11-28
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pubmed:abstractText |
Sau3AI is a type II restriction enzyme that recognizes the 5'-GATC-3' sequence in double-strand DNA and cleaves at 5' to the G residue. The C-terminal domain of Sau3AI (Sau3AI-C), which contains amino acids from 233 to 489, was crystallized and its structure was solved by using the Multi-wavelength Anomalous Diffraction method. The Sau3AI-C structure at 1.9 A resolution is similar to the structure of MutH, a DNA mismatch repair protein that shares high sequence similarity with the N-terminal Sau3AI domain. The functional analysis shows that Sau3AI-C can bind DNA with one recognition sequence but has no cleavage activity. These results indicate that Sau3AI is a pseudo-dimer belonging to the type IIe restriction enzymes and the Sau3AI-C is the allosteric effector domain that assists DNA binding and cleavage.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1794
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
118-23
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pubmed:meshHeading |
pubmed-meshheading:18930848-Amino Acid Sequence,
pubmed-meshheading:18930848-Base Pair Mismatch,
pubmed-meshheading:18930848-Binding Sites,
pubmed-meshheading:18930848-Crystallography, X-Ray,
pubmed-meshheading:18930848-DNA Repair,
pubmed-meshheading:18930848-DNA-Binding Proteins,
pubmed-meshheading:18930848-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:18930848-Models, Molecular,
pubmed-meshheading:18930848-Molecular Sequence Data,
pubmed-meshheading:18930848-Peptide Fragments,
pubmed-meshheading:18930848-Protein Structure, Tertiary,
pubmed-meshheading:18930848-Sequence Homology, Amino Acid
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pubmed:year |
2009
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pubmed:articleTitle |
Crystal structure and function of C-terminal Sau3AI domain.
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pubmed:affiliation |
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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