rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
25
|
pubmed:dateCreated |
1991-10-4
|
pubmed:databankReference |
|
pubmed:abstractText |
A functional cloning procedure has been used to recover a cDNA coding for the GMP synthetase of Dictyostelium discoideum. The enzyme is encoded by a single gene, which is actively transcribed during growth, but not during development. The open reading frame encodes a protein of 718 amino acids with a predicted molecular mass of 79.6 kDa. The Dictyostelium enzyme has extensive homology with the GMP synthetase of Escherichia coli and regional homology to other glutamine amidotransferases.
|
pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0021-9258
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
5
|
pubmed:volume |
266
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
16448-52
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:1885577-Amino Acid Sequence,
pubmed-meshheading:1885577-Base Sequence,
pubmed-meshheading:1885577-Blotting, Northern,
pubmed-meshheading:1885577-Blotting, Southern,
pubmed-meshheading:1885577-Carbon-Nitrogen Ligases,
pubmed-meshheading:1885577-Cloning, Molecular,
pubmed-meshheading:1885577-DNA, Fungal,
pubmed-meshheading:1885577-Dictyostelium,
pubmed-meshheading:1885577-Escherichia coli,
pubmed-meshheading:1885577-Ligases,
pubmed-meshheading:1885577-Molecular Sequence Data,
pubmed-meshheading:1885577-Sequence Alignment
|
pubmed:year |
1991
|
pubmed:articleTitle |
Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase.
|
pubmed:affiliation |
Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|