Source:http://linkedlifedata.com/resource/pubmed/id/18848913
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2008-11-17
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pubmed:abstractText |
Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI-TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ALDH1A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Gelsolin,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1874-3919
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
561-71
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pubmed:dateRevised |
2010-10-13
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pubmed:meshHeading |
pubmed-meshheading:18848913-Aldehyde Dehydrogenase,
pubmed-meshheading:18848913-Cell Line, Tumor,
pubmed-meshheading:18848913-Clone Cells,
pubmed-meshheading:18848913-Gelsolin,
pubmed-meshheading:18848913-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18848913-Humans,
pubmed-meshheading:18848913-Neoplasm Invasiveness,
pubmed-meshheading:18848913-Neoplasm Proteins,
pubmed-meshheading:18848913-Pancreatic Neoplasms,
pubmed-meshheading:18848913-RNA, Neoplasm,
pubmed-meshheading:18848913-RNA, Small Interfering
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pubmed:year |
2008
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pubmed:articleTitle |
Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells.
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pubmed:affiliation |
National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. naomi.walsh@dcu.ie
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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