Linked Life Data

1884200

Source:http://linkedlifedata.com/resource/pubmed/id/1884200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-10-4
pubmed:abstractText
Clusters of carotid body (glomus) cells cultured from a few days to 3 weeks, maintained their morphological characteristics during this period. The resting potential (Em) and input resistance (Ro) did not change for 2 weeks but both declined afterwards. The intracellular pH (pHi) of glomus cells, measured with glass microelectrodes filled with an H+ ion exchanger, was 6.34-6.96 at extracellular pH (pHo) of 7.32-7.53. Changes in pHo from normal to about 5.5 depolarized most cells but the fall in pHi was less marked than predicted by the Nernst equation. Conversely, shifting pHo to 8.5 hyperpolarized the cells with an increase in pHi which was more acid than predicted. EH (the calculated equilibrium potential for H+ (Nernst equation) was more positive than Em during acidity and more negative during normal or alkaline pHo. The kinetics of H+ ion distribution was assessed by brief exposures to NH4Cl. It is concluded that hydrogen ions are not passively distributed across the glomus cell membranes and that Em is dependent on H+ ions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
547
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
258-66
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Intracellular pH and some membrane characteristics of cultured carotid body glomus cells.
pubmed:affiliation
Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't