Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
2008-10-16
pubmed:abstractText
Polyspermy is an important anomaly of fertilization in placental mammals, causing premature death of the embryo. It is especially frequent under in vitro conditions, complicating the successful generation of viable embryos. A block to polyspermy develops as a result of changes after sperm entry (i.e., cortical granule exocytosis). However, additional factors may play an important role in regulating polyspermy by acting on gametes before sperm-oocyte interaction. Most studies have used rodents as models, but ungulates may differ in mechanisms preventing polyspermy. We hypothesize that zona pellucida (ZP) changes during transit of the oocyte along the oviductal ampulla modulate the interaction with spermatozoa, contributing to the regulation of polyspermy. We report here that periovulatory oviductal fluid (OF) from sows and heifers increases (both, con- and heterospecifically) ZP resistance to digestion with pronase (a parameter commonly used to measure the block to polyspermy), changing from digestion times of approximately 1 min (pig) or 2 min (cattle) to 45 min (pig) or several hours (cattle). Exposure of oocytes to OF increases monospermy after in vitro fertilization in both species, and in pigs, sperm-ZP binding decreases. The resistance of OF-exposed oocytes to pronase was abolished by exposure to heparin-depleted medium; in a medium with heparin it was not altered. Proteomic analysis of the content released in the heparin-depleted medium after removal of OF-exposed oocytes allowed the isolation and identification of oviduct-specific glycoprotein. Thus, an oviduct-specific glycoprotein-heparin protein complex seems to be responsible for ZP changes in the oviduct before fertilization, affecting sperm binding and contributing to the regulation of polyspermy.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-10690208, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-10732734, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-10859265, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-11302427, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-11882012, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-11988322, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-12141941, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-12748122, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-14694441, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-14695374, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-15191231, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-15376919, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-15474476, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-15615895, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-15652185, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-15763112, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-16098694, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-16493683, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-16726723, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-16870945, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-17644974, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-18159080, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-1888518, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-2104813, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-2297082, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-2374118, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-2492959, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-2496140, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-2661516, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-270665, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-3171167, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-3209175, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-4038732, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-4462855, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-4938367, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-8318581, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-8699438, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-8949888, http://linkedlifedata.com/resource/pubmed/commentcorrection/18838686-9491383
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
14
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15809-14
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during fertilization and contribute to the control of polyspermy.
pubmed:affiliation
Physiology of Reproduction Group, Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia, Murcia 30071, Spain. pcoy@um.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't