Linked Life Data

18828837

Source:http://linkedlifedata.com/resource/pubmed/id/18828837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-10-2
pubmed:abstractText
Spider dragline silk is primarily composed of proteins called major ampullate spidroins (MaSps) that consist of a large repeat array flanked by nonrepetitive N- and C-terminal domains. Until recently, there has been little evidence for more than one gene encoding each of the two major spidroin silk proteins, MaSp1 and MaSp2. Here, we report the deduced N-terminal domain sequences for two distinct MaSp1 genes from Nephila clavipes (MaSp1A and MaSp1B) and for MaSp2. All three MaSp genes are co-expressed in the major ampullate gland. A search of the GenBank database also revealed two distinct MaSp1 C-terminal domain sequences. Sequencing confirmed that both MaSp1 genes are present in all seven Nephila clavipes spiders examined. The presence of nucleotide polymorphisms in these genes confirmed that MaSp1A and MaSp1B are distinct genetic loci and not merely alleles of the same gene. We experimentally determined the transcription start sites for all three MaSp genes and established preliminary pairing between the two MaSp1 N- and C-terminal domains. Phylogenetic analysis of these new sequences and other published MaSp N- and C-terminal domain sequences illustrated that duplications of MaSp genes may be widespread among spider species.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10328265, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10342774, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10342779, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10562512, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10592250, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10694887, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-10828366, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-11112271, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-11283372, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-11480706, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-11765852, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-12165842, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15132670, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15223320, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-1527052, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15491167, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15556231, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15779899, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15795489, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-15971267, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-16097721, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-16253207, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-16283740, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-16768399, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-16788028, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-16967919, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-17096540, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-17402782, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-17488738, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-17565367, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-17577991, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-17680798, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-18048404, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-18293938, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-2402494, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-465570, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-8120021, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-8125298, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-8968613, http://linkedlifedata.com/resource/pubmed/commentcorrection/18828837-9480768
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1365-2583
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-74
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes.
pubmed:affiliation
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't