Linked Life Data

18785172

Source:http://linkedlifedata.com/resource/pubmed/id/18785172

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-11
pubmed:abstractText
This unit describes the derivation of pancreatic cells from mouse embryonic stem cells (ESCs). Mouse ESCs are pluripotent immortal cells derived from the inner cell mass of pre-implantation blastocyst-stage embryos that possess the ability to differentiate into any cell type within the adult animal. In vitro, ESCs can be differentiated into a variety of cell types representing derivatives of the three embryonic germ layers, mesoderm, endoderm, and ectoderm. Successfully differentiating ES cells to pancreatic cells has the potential to provide an alternative to cadaver-derived cells for treatment of type I diabetes. This unit outlines a method for the differentiation of ESCs toward pancreatic endoderm in serum-free medium from embryoid bodies (EBs) formed in suspension or spin EBs. In addition there is a protocol for maintaining ESC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1938-8969
pubmed:author
pubmed:copyrightInfo
Copyright 2007 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 1G.2
pubmed:dateRevised
2009-12-11
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Pancreas differentiation of mouse ES cells.
pubmed:affiliation
Monash University, Victoria, Australia.
pubmed:publicationType
Journal Article