Linked Life Data

18770720

Source:http://linkedlifedata.com/resource/pubmed/id/18770720

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-4
pubmed:abstractText
Study of dendritic cells is complicated by their low frequency and the lack of specific markers for sensitive and specific identification. Furthermore, these cells are not a homogeneous population, but contain a variety of subsets. Flow cytometry has well-established applications for the analysis of cells present at low frequencies; the use of a two-step acquisition procedure increases the number of cells for analysis. This unit presents protocols for the identification and enumeration of dendritic cells and cell subsets in erythrocyte-lysed whole peripheral blood. Special attention is paid to the combinations of reagents used for the identification of all dendritic cells as well as each subset, and on the multiparametric gating strategies for specific analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1934-9300
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 6.9
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Immunophenotypic identification, enumeration, and characterization of human peripheral blood dendritic cells and dendritic-cell precursors.
pubmed:affiliation
Universidad de Salamanca, Salamanca, Spain.
pubmed:publicationType
Journal Article