Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-4
pubmed:abstractText
This unit presents protocols for counting cells using either a hemacytometer or electronically using a Coulter counter. Cell counting with a hemacytometer permits effective discrimination of live from dead cells using trypan blue exclusion. In addition, the procedure is less subject to errors arising from cell clumping or size heterogeneity. Counting cells is more quickly and easily performed using an electronic counter, but live-dead discrimination is unreliable. Cell populations containing large numbers of dead cells and/or cell clumps are difficult to count accurately. In addition, electronic counting requires resetting of the instrument for cell populations of different sizes; heterogeneous populations can give rise to inaccurate counts, and resting and activated cells may require counting at separate settings. In general, electronic cell counting is best performed on fresh peripheral blood cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-9300
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Appendix 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Appendix 3A
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Cell counting.
pubmed:affiliation
Thompson Children's Hospital, Chattanooga, Tennessee, USA.
pubmed:publicationType
Journal Article