Linked Life Data

18770557

Source:http://linkedlifedata.com/resource/pubmed/id/18770557

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-9-4
pubmed:abstractText
The prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, Sindbis virus shuttles from an insect vector to a vertebrate host and back to the insect vector in a complex transmission cycle. Sindbis virus must, therefore, be able to replicate in two biochemically and genetically divergent hosts, invertebrates and vertebrates. In the laboratory, Sindbis grows to high titers in a large variety of cultured cells of both vertebrate and invertebrate origin. Sindbis virus is easily titered for infectivity on several mammalian cell lines as well as certain mosquito cells. Full-length cDNA clones of several strains of Sindbis virus are available from which infectious RNA can be synthesized, making possible the genetic manipulation of the virus. Transfection of mammalian and insect cells by electroporation has facilitated expression of RNA mutants in the cell lines of choice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1934-8533
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 15B.1
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Sindbis virus: propagation, quantification, and storage.
pubmed:affiliation
North Carolina State University, Raleigh, North Carolina, USA.
pubmed:publicationType
Journal Article