Linked Life Data

18770014

Source:http://linkedlifedata.com/resource/pubmed/id/18770014

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-10-29
pubmed:abstractText
We investigated the age-related alterations of calcineurin and Akt1/protein kinase Balpha (Akt1/PKBalpha) immunoreactivity in the mouse hippocampal CA1 sector using immunohistochemistry. Calcineurin and Akt1/PKBalpha immunoreactivity was measured in 2-, 8-, 18-, 40-42- and 50-59-weeks-old animals. Diffuse calcineurin immunoreactivity was evident in pyramidal neurons of the hippocampal CA1 sector of 8-weeks-old mice. Densities of calcineurin immunoreactivity were lowered significantly in the hippocampal CA1 neurons of 2-weeks-old mice. In contrast, densities of calcineurin immunoreactivity were unchanged in the hippocampal CA1 neurons up to 40-42-weeks-old mice. However, densities of calcineurin immunoreactivity were increased significantly in the dendrites and plasma membranes of the hippocampal CA1 neurons of 50-59-weeks-old mice compared to 8-weeks old animals. Akt1/PKBalpha immunoreactivity was slightly detectable in the hippocampal CA1 sector of 8-weeks-old mice. A weak Akt1/PKBalpha immunoreactivity was found in cytoplasm of the hippocampal CA1 neurons and glial cells. Densities of Akt1/PKBalpha immunoreactivity were unchanged in the hippocampal CA1 neurons and glial cells of 2-weeks-old mice. In contrast, densities of Akt1/PKBalpha immunoreactivity were increased significantly in cytoplasm of neurons and glial cells of the hippocampal CA1 sector from 40-42 to 50-59 weeks after birth. The present study indicates that densities of calcineurin immunoreactivity and number of Akt1/PKBalpha immunoreactive cells were increased significantly in the hippocampal CA1 sector during aging processes. Our study also demonstrates that the activation of Akt1/PKBalpha signaling pathway may act defense mechanism against the neuronal dysfunction of the hippocampal CA1 sector caused by the activation of calcineurin signaling pathway during aging processes. These findings suggest that the calcineurin and Akt1/PKBalpha signaling pathway may be important targets for the development of novel therapeutic strategies for protection against age-related neurodegeneration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0885-7490
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
399-409
pubmed:dateRevised
2011-7-14
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Age-related changes of calcineurin and Akt1/protein kinase Balpha (Akt1/PKBalpha) immunoreactivity in the mouse hippocampal CA1 sector: an immunohistochemical study.
pubmed:affiliation
Department of Neurobiology and Therapeutics, Graduate School and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78, Sho-machi, Tokushima, 770-8505, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't