Linked Life Data

18763703

Source:http://linkedlifedata.com/resource/pubmed/id/18763703

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2008-10-1
pubmed:abstractText
The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples. Among protein samples, membrane proteins are one of the most difficult sample classes, because of their hydrophobicity and embedment in the lipid bilayers. This review deals with the recent progresses and advances made in the separation of membrane proteins by 2-DE separating only denatured proteins. Traditional 2-D methods, i.e., methods using IEF in the first dimension are compared to methods using only zone electrophoresis in both dimensions, i.e., electrophoresis in the presence of cationic or anionic detergents. The overall performances and fields of application of both types of method is critically examined, as are future prospects for this field.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1615-9861
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3965-73
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update.
pubmed:affiliation
CEA-DSV/iRTSV/LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA-Grenoble, Grenoble, France. thierry.rabilloud@cea.fr
pubmed:publicationType
Journal Article, Review