| pubmed-article:18694522 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C1638187 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C0086661 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C0007102 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C0754515 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C1099354 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:18694522 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:18694522 | pubmed:dateCreated | 2008-8-29 | lld:pubmed |
| pubmed-article:18694522 | pubmed:abstractText | The c-Myc and human telomerase reverse transcriptase gene (hTERT) gene are frequently deregulated and overexpressed in malignancy. hTERT activity is induced by c-Myc and strategies designed to inhibit c-Myc expression in cancer cells may have considerable therapeutic value. We designed and used a short hairpin RNA to inhibit c-Myc expression in Colo 320 cells and validated its effect on cell proliferation. In this study, four c-Myc-shRNA expression vectors were constructed and introduced into Colo 320 cells. The effects of c-Myc silencing on tumor cell growth was assessed by soft agar assay and DNA synthesis experiments. The expressions of c-Myc and hTERT were also assessed by real-time reverse transcription-polymerase chain reaction and Western blot analysis. Upon transient transfection with plasmid encoding shRNA, it was found that expression of c-Myc and hTERT decreased in shRNA-transfected cells. The downregulation of c-Myc and hTERT inhibited cell growth, shortened telomere lengths, and suppressed telomerase activity. In conclusion, our findings demonstrate that shRNA of c-Myc can inhibit the DNA replication in Colo 320 cells effectively and reduce telomere length and telomerase activity, therefore, it could be used as a new potential anticancer tool for therapy of human colon cancer. | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18694522 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18694522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18694522 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18694522 | pubmed:issn | 1756-9966 | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:SeckGG | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:NanasiPP | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:XiaoHongH | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:HuangHaoH | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:HuangGuofuG | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:XiongWeiW | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:FuLeiL | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:SuWenW | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:HuangHanjuH | lld:pubmed |
| pubmed-article:18694522 | pubmed:author | pubmed-author:WuYanxiaY | lld:pubmed |
| pubmed-article:18694522 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18694522 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:18694522 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18694522 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18694522 | pubmed:pagination | 27 | lld:pubmed |
| pubmed-article:18694522 | pubmed:dateRevised | 2010-1-21 | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:meshHeading | pubmed-meshheading:18694522... | lld:pubmed |
| pubmed-article:18694522 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18694522 | pubmed:articleTitle | siRNA directed against c-Myc inhibits proliferation and downregulates human telomerase reverse transcriptase in human colon cancer Colo 320 cells. | lld:pubmed |
| pubmed-article:18694522 | pubmed:affiliation | Center of Experimental Medicine, Wuhan No,1 Hospital, Wuhan, 430022, PR China. hao4@163.com | lld:pubmed |
| pubmed-article:18694522 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18694522 | pubmed:publicationType | Retracted Publication | lld:pubmed |
| pubmed-article:18694522 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18694522 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18694522 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18694522 | lld:pubmed |
