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rdf:type |
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lifeskim:mentions |
umls-concept:C0007102,
umls-concept:C0007634,
umls-concept:C0086418,
umls-concept:C0086661,
umls-concept:C0439851,
umls-concept:C0754515,
umls-concept:C1099354,
umls-concept:C1514485,
umls-concept:C1552596,
umls-concept:C1638187,
umls-concept:C1947931
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pubmed:dateCreated |
2008-8-29
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pubmed:abstractText |
The c-Myc and human telomerase reverse transcriptase gene (hTERT) gene are frequently deregulated and overexpressed in malignancy. hTERT activity is induced by c-Myc and strategies designed to inhibit c-Myc expression in cancer cells may have considerable therapeutic value. We designed and used a short hairpin RNA to inhibit c-Myc expression in Colo 320 cells and validated its effect on cell proliferation. In this study, four c-Myc-shRNA expression vectors were constructed and introduced into Colo 320 cells. The effects of c-Myc silencing on tumor cell growth was assessed by soft agar assay and DNA synthesis experiments. The expressions of c-Myc and hTERT were also assessed by real-time reverse transcription-polymerase chain reaction and Western blot analysis. Upon transient transfection with plasmid encoding shRNA, it was found that expression of c-Myc and hTERT decreased in shRNA-transfected cells. The downregulation of c-Myc and hTERT inhibited cell growth, shortened telomere lengths, and suppressed telomerase activity. In conclusion, our findings demonstrate that shRNA of c-Myc can inhibit the DNA replication in Colo 320 cells effectively and reduce telomere length and telomerase activity, therefore, it could be used as a new potential anticancer tool for therapy of human colon cancer.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-10591218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-10648922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-10786671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-11175856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-11274400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-11376932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-11884529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-11972060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-12149476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-12360279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-12538578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-14563552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-14663479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-14685090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-14966287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-15094035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-15314163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-15372045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-15517017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-19607687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-2805070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-7605428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-8934879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-9256460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18694522-9486653
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1756-9966
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27
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pubmed:dateRevised |
2010-1-21
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pubmed:meshHeading |
pubmed-meshheading:18694522-Cell Line, Tumor,
pubmed-meshheading:18694522-Cell Proliferation,
pubmed-meshheading:18694522-Colonic Neoplasms,
pubmed-meshheading:18694522-Down-Regulation,
pubmed-meshheading:18694522-Humans,
pubmed-meshheading:18694522-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:18694522-RNA, Small Interfering,
pubmed-meshheading:18694522-RNA Interference,
pubmed-meshheading:18694522-Telomerase,
pubmed-meshheading:18694522-Telomere,
pubmed-meshheading:18694522-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
siRNA directed against c-Myc inhibits proliferation and downregulates human telomerase reverse transcriptase in human colon cancer Colo 320 cells.
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pubmed:affiliation |
Center of Experimental Medicine, Wuhan No,1 Hospital, Wuhan, 430022, PR China. hao4@163.com
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pubmed:publicationType |
Journal Article,
Retracted Publication,
Research Support, Non-U.S. Gov't
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