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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 8
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pubmed:dateCreated |
2008-8-5
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pubmed:abstractText |
Transfer RNA (tRNA) (m(7)G46) methyltransferase (TrmB) belongs to the Rossmann-fold methyltransferase (RFM) family and uses S-adenosyl-L-methionine (SAM) as the methyl-group donor to catalyze the formation of N(7)-methylguanosine (m(7)G) at position 46 in the variable loop of tRNAs. After attempts to crystallize full-length Escherichia coli TrmB (EcTrmB) failed, a truncated protein lacking the first 32 residues of the N-terminus but with an additional His(6) tag at the C-terminus was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 (PEG 3350) as precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group P2(1).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-10376676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-12403464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-12730187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-15811913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-15947016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-16600901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-16682782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-18029735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-18184583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-9399834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678947-9847178
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
743-5
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pubmed:dateRevised |
2010-9-21
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Crystallization and preliminary crystallographic analysis of tRNA (m(7)G46) methyltransferase from Escherichia coli.
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pubmed:affiliation |
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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