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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-1-30
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pubmed:abstractText |
MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1530-0285
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pubmed:author |
pubmed-author:AherneSinead TST,
pubmed-author:AlhadiAraibiA,
pubmed-author:AzizNatasha ANA,
pubmed-author:BarrettCiaraC,
pubmed-author:DenningKaren MKM,
pubmed-author:FinnStephen PSP,
pubmed-author:FlavinRichard JRJ,
pubmed-author:LaiosAlexandrosA,
pubmed-author:LewM JMJ,
pubmed-author:LiJinghuanJ,
pubmed-author:O'LearyJohn JJJ,
pubmed-author:O'TooleSharon ASA,
pubmed-author:RingMartinaM,
pubmed-author:RussellSusanS,
pubmed-author:SammaraeDania ADA,
pubmed-author:SheilsOrla MOM,
pubmed-author:SheppardBrian LBL,
pubmed-author:SmythPaul CPC
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pubmed:issnType |
Electronic
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
197-205
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pubmed:meshHeading |
pubmed-meshheading:18677302-Adult,
pubmed-meshheading:18677302-Aged,
pubmed-meshheading:18677302-Aged, 80 and over,
pubmed-meshheading:18677302-Carcinoma,
pubmed-meshheading:18677302-Chromosomes, Human, Pair 17,
pubmed-meshheading:18677302-Down-Regulation,
pubmed-meshheading:18677302-Female,
pubmed-meshheading:18677302-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18677302-Humans,
pubmed-meshheading:18677302-Immunohistochemistry,
pubmed-meshheading:18677302-MicroRNAs,
pubmed-meshheading:18677302-Middle Aged,
pubmed-meshheading:18677302-Ovarian Neoplasms,
pubmed-meshheading:18677302-Peritoneal Neoplasms,
pubmed-meshheading:18677302-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:18677302-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18677302-Tissue Array Analysis,
pubmed-meshheading:18677302-Tumor Suppressor Protein p53
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pubmed:year |
2009
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pubmed:articleTitle |
Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma.
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pubmed:affiliation |
Department of Histopathology, Trinity College Dublin, Dublin, Ireland. flavinr@tcd.ie
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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