Linked Life Data

18639174

Source:http://linkedlifedata.com/resource/pubmed/id/18639174

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-7-21
pubmed:abstractText
Four proteins unique to virus infection were synthesized in alfalfa mosaic virus-infected alfalfa mesophyll protoplasts. These proteins, P1, P2, P3, and coat protein comigrated on electrophoresis with the major in vitro translation products of RNA 1, RNA 2, RNA 3, and RNA 4, respectively. P1, P3, and coat protein were observed at 5 hr post inoculation; P2 was detected at 9 hr post inoculation. The three nonstructural proteins accumulated most rapidly early in infection until about 15 hr post inoculation; stable protein levels were maintained thereafter. Coat protein accumulated rapidly until about 20 hr after inoculation. All four virus RNA species were detected in infected protoplasts by labelling with [3H]uridine. Ultraviolet irradiation of protoplasts prior to inoculation was necessary for virus protein detection, but it severely depressed the synthesis of RNA 1 and RNA 2 relative to RNA 3 and RNA 4.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Dec
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
455-62
pubmed:year
1983
pubmed:articleTitle
Virus protein synthesis in alfalfa mosaic virus infected alfalfa protoplasts.
pubmed:affiliation
Agrigenetics Advanced Research Laboratory, 5649 East Buckeye Road, Madison, Wisconsin 53716, USA.
pubmed:publicationType
Journal Article