Linked Life Data

18631642

Source:http://linkedlifedata.com/resource/pubmed/id/18631642

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-7-17
pubmed:abstractText
RNAs of pepper mottle virus (PeMV) and tobacco etch virus (TEV) were efficient messengers when translated in the rabbit reticulocyte lysate system. Viral RNA (39 S) isolated from sucrose density gradients stimulated [35S]methionine incorporation into products precipitated by trichloroacetic acid 15- to 20-fold over endogenous background levels. Optimal reaction conditions for in vitro translation contained 2 mug RNA/30 mul reaction assay, 0.8-1.0 mM magnesium ions, and 100-125 mM potassium ions. Labeled products from the translation of TEV and PeMV show distinct electrophoretic patterns on sodium dodecyl sulfate polyacrylamide gradient gels (PAGE). The major products of TEV translation had estimated sizes of 87 x 10(4) daltons (87 kd), 85, 54, 49, and 30 kd. PeMV-RNA stimulated the synthesis of 90-, 77-, 68-, 49-, and 33-kd proteins. The 30-kd protein for TEV and the 33-kd protein for PeMV were identified as capsid protein on the basis of estimated size on PAGE, serological reaction, and peptide mapping. The strategy of the in vitro translation of potyvirus RNA is discussed.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Mar
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
466-74
pubmed:year
1980
pubmed:articleTitle
Translation of potyvirus RNA in a rabbit reticulocyte lysate: reaction conditions and identification of capsid protein as one of the products of in vitro translation of tobacco etch and pepper mottle viral RNAs.
pubmed:affiliation
Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, USA.
pubmed:publicationType
Journal Article