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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2008-7-11
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pubmed:abstractText |
Noise in the expression of a gene produces fluctuations in the concentration of the gene product. These fluctuations can interfere with optimal function or can be exploited to generate beneficial diversity between cells; gene expression noise is therefore expected to be subject to evolutionary pressure. Shifts between modes of high and low rates of transcription initiation at a promoter appear to contribute to this noise both in eukaryotes and prokaryotes. However, models invoked for eukaryotic promoter noise such as stable activation scaffolds or persistent nucleosome alterations seem unlikely to apply to prokaryotic promoters. We consider the relative importance of the steps required for transcription initiation. The 3-step transcription initiation model of McClure is extended into a mathematical model that can be used to predict consequences of additional promoter properties. We show in principle that the transcriptional bursting observed at an E. coli promoter by Golding et al. (2005) can be explained by stimulation of initiation by the negative supercoiling behind a transcribing RNA polymerase (RNAP) or by the formation of moribund or dead-end RNAP-promoter complexes. Both mechanisms are tunable by the alteration of promoter kinetics and therefore allow the optimization of promoter mediated noise.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18617999-10364545,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18617999-9809065
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1553-7358
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e1000109
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:18617999-DNA, Superhelical,
pubmed-meshheading:18617999-DNA-Directed RNA Polymerases,
pubmed-meshheading:18617999-Escherichia coli,
pubmed-meshheading:18617999-Gene Expression Regulation, Bacterial,
pubmed-meshheading:18617999-Kinetics,
pubmed-meshheading:18617999-Models, Genetic,
pubmed-meshheading:18617999-Promoter Regions, Genetic,
pubmed-meshheading:18617999-Transcription, Genetic,
pubmed-meshheading:18617999-Transcription Factors
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pubmed:year |
2008
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pubmed:articleTitle |
The generation of promoter-mediated transcriptional noise in bacteria.
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pubmed:affiliation |
Department of Physics, Kyushu University, Fukuoka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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