Source:http://linkedlifedata.com/resource/pubmed/id/18616406
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
2008-7-11
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pubmed:abstractText |
The quorum-sensing (QS) regulatory system of the gall-forming Pantoea agglomerans pv. gypsophilae was identified. Mass spectral analysis, together with signal-specific biosensors, demonstrated that P. agglomerans pv. gypsophilae produced N-butanoyl-l-homoserine lactone (C4-HSL) as a major and N-hexanoyl-l-homoserine lactone (C6-HSL) as a minor QS signal. Homologs of luxI and luxR regulatory genes, pagI and pagR, were characterized in strain P. agglomerans pv. gypsophilae Pag824-1 and shown to be convergently transcribed and separated by 14 bp. The deduced PagI (23.8 kDa) and PagR (26.9 kDa) show high similarity with SmaI (41% identity) and SmaR (43% identity), respectively, of Serratia sp. American Type Culture Collection 39006. PagR possesses characteristic autoinducer binding and a helix-turn-helix DNA-binding domain. Gall formation by P. agglomerans pv. gypsophilae depends on a plasmid-borne hrp/hrc gene cluster, type III effectors, and phytohormones. Disruption of pagI, pagR, or both genes simultaneously in Pag824-1 reduced gall size in gypsophila cuttings by 50 to 55% when plants were inoculated with 10(6) CFU/ml. Higher reductions in gall size (70 to 90%) were achieved by overexpression of pagI or addition of exogenous C4-HSL. Expression of the hrp/hrc regulatory gene hrpL and the type III effector pthG in the pagI mutant, as measured with quantitative reverse-transcriptase polymerase chain reaction, was reduced by 5.8 and 6.6, respectively, compared with the wild type, suggesting an effect of the QS system on the Hrp regulon.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-Butyrolactone,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LuxI protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/LuxR autoinducer binding proteins,
http://linkedlifedata.com/resource/pubmed/chemical/N-butyrylhomoserine lactone,
http://linkedlifedata.com/resource/pubmed/chemical/N-hexanoyl-L-homoserine lactone,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0894-0282
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1094-105
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pubmed:meshHeading |
pubmed-meshheading:18616406-4-Butyrolactone,
pubmed-meshheading:18616406-Amino Acid Sequence,
pubmed-meshheading:18616406-Bacterial Proteins,
pubmed-meshheading:18616406-Caryophyllaceae,
pubmed-meshheading:18616406-Cloning, Molecular,
pubmed-meshheading:18616406-Gene Expression Regulation, Bacterial,
pubmed-meshheading:18616406-Genes, Bacterial,
pubmed-meshheading:18616406-Molecular Sequence Data,
pubmed-meshheading:18616406-Pantoea,
pubmed-meshheading:18616406-Plant Tumors,
pubmed-meshheading:18616406-Quorum Sensing,
pubmed-meshheading:18616406-Repressor Proteins,
pubmed-meshheading:18616406-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18616406-Trans-Activators,
pubmed-meshheading:18616406-Transcription Factors
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pubmed:year |
2008
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pubmed:articleTitle |
Quorum-sensing system affects gall development incited by Pantoea agglomerans pv. gypsophilae.
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pubmed:affiliation |
Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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