Linked Life Data

1860846

Source:http://linkedlifedata.com/resource/pubmed/id/1860846

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1991-9-4
pubmed:databankReference
pubmed:abstractText
The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types. We have begun to characterize the human gene in order to elucidate the genetic elements responsible for this highly regulated expression. We first cloned a human MARCKS cDNA, which encoded a predicted protein of 332 amino acids (Mr 31,600) that was approximately 89, 74, and 59% identical to the bovine, mouse, and chicken proteins, respectively. Regions conserved at the amino acid level included the amino-terminal myristoylation consensus sequence, the site of intron splicing, and the phosphorylation site domain. The human cDNA was used to demonstrate that tumor necrosis factor-alpha could rapidly stimulate MARCKS gene transcription in the human promyelocytic leukemia cell line HL60. Genomic clones were then isolated; sequence analysis identified a putative promoter region that had no TATA box and contained multiple transcription initiation sites in a region spanning 57 base pairs (bp). This was followed by a 5'-untranslated region of approximately 400 bp, which displayed a complex predicted secondary structure with a delta G of -73.4 kcal/mol. Plasmid constructions containing between 52 and 1453 bp of the human MARCKS promoter linked to the human growth hormone gene were then used in transient expression experiments. Constructions containing 52 and 110 bp of the MARCKS promoter did not exhibit promoter function while the larger constructions all exhibited promotor function; the 248-bp fragment of the MARCKS promoter was 80% as effective as the human ferritin promoter in stimulating expression of human growth hormone in intact cells. Using an insert from the human genomic clone as a probe, we identified human chromosome 6, q21-qter, as the location of the MARCKS gene; this has been assigned the gene symbol MACS.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
266
pubmed:geneSymbol
MACS
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14399-405
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1860846-Amino Acid Sequence, pubmed-meshheading:1860846-Animals, pubmed-meshheading:1860846-Base Sequence, pubmed-meshheading:1860846-Blotting, Northern, pubmed-meshheading:1860846-Cattle, pubmed-meshheading:1860846-Chickens, pubmed-meshheading:1860846-Chromosome Mapping, pubmed-meshheading:1860846-Chromosomes, Human, Pair 6, pubmed-meshheading:1860846-DNA, pubmed-meshheading:1860846-Humans, pubmed-meshheading:1860846-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:1860846-Membrane Proteins, pubmed-meshheading:1860846-Mice, pubmed-meshheading:1860846-Molecular Sequence Data, pubmed-meshheading:1860846-Nucleic Acid Conformation, pubmed-meshheading:1860846-Plasmids, pubmed-meshheading:1860846-Promoter Regions, Genetic, pubmed-meshheading:1860846-Protein Kinase C, pubmed-meshheading:1860846-Proteins, pubmed-meshheading:1860846-RNA, Messenger, pubmed-meshheading:1860846-Sequence Alignment, pubmed-meshheading:1860846-Sequence Homology, Nucleic Acid
pubmed:year
1991
pubmed:articleTitle
The human myristoylated alanine-rich C kinase substrate (MARCKS) gene (MACS). Analysis of its gene product, promoter, and chromosomal localization.
pubmed:affiliation
Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't