Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-7-3
pubmed:abstractText
Microtubules are a major structural component of the cytoskeleton and participate in cell division, intracellular transport, and cell morphogenesis. In the present study, 795 cotton tubulin expressed sequence tags were analysed and 19 beta-tubulin genes (TUB) cloned from a cotton cDNA library. Among the group, 12 cotton TUBs (GhTUBs) are reported for the first time here. Transcription profiling revealed that nine GhTUBs were highly expressed in elongating fibre cells as compared with fuzzless-lintless mutant ovules. Treating cultured wild-type cotton ovules with exogenous phytohormones showed that individual genes can be induced by different agents. Gibberellin induced expression of GhTUB1 and GhTUB3, ethylene induced expression of GhTUB5, GhTUB9, and GhTUB12, brassinosteroids induced expression of GhTUB1, GhTUB3, GhTUB9, and GhTUB12, and lignoceric acid induced expression of GhTUB1, GhTUB3, and GhTUB12. When GhTUBs were transformed into the Saccharomyces cerevisiae inviable mutant, tub2, which is deficient in beta-tubulin, one ovule-specific and eight of nine fibre-preferential GhTUBs rescued this lethality. This study suggests that the proteins encoded by cotton GhTUBs are involved during cotton fibre development.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-10230063, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-10482673, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-10760237, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-11385579, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-11587510, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-11722758, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-11743074, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-12376634, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-12736302, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-14634157, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-1498608, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-1498609, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-1522605, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-15260895, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-15604659, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-1581978, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-16461577, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-16627697, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-16698945, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17122407, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-1713800, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17337629, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17387330, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17567681, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17761728, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17873093, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17885081, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17905721, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-17993622, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-1912487, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-2998923, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-8111033, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-8148650, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-8186463, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-8994660, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-9428769, http://linkedlifedata.com/resource/pubmed/commentcorrection/18596112-9881152
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1460-2431
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2687-95
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
pubmed-meshheading:18596112-Amino Acid Sequence, pubmed-meshheading:18596112-Cloning, Molecular, pubmed-meshheading:18596112-DNA, Complementary, pubmed-meshheading:18596112-Fatty Acids, pubmed-meshheading:18596112-Gene Expression Profiling, pubmed-meshheading:18596112-Gene Expression Regulation, Plant, pubmed-meshheading:18596112-Gene Library, pubmed-meshheading:18596112-Genetic Complementation Test, pubmed-meshheading:18596112-Gossypium, pubmed-meshheading:18596112-Molecular Sequence Data, pubmed-meshheading:18596112-Phylogeny, pubmed-meshheading:18596112-Plant Growth Regulators, pubmed-meshheading:18596112-Plant Proteins, pubmed-meshheading:18596112-Saccharomyces cerevisiae, pubmed-meshheading:18596112-Sequence Alignment, pubmed-meshheading:18596112-Tubulin, pubmed-meshheading:18596112-Up-Regulation
pubmed:year
2008
pubmed:articleTitle
Molecular cloning, expression profiling, and yeast complementation of 19 beta-tubulin cDNAs from developing cotton ovules.
pubmed:affiliation
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't