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rdf:type |
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lifeskim:mentions |
umls-concept:C0015127,
umls-concept:C0017262,
umls-concept:C0026882,
umls-concept:C0162741,
umls-concept:C0185117,
umls-concept:C0205099,
umls-concept:C0242728,
umls-concept:C0243067,
umls-concept:C1314792,
umls-concept:C1332381,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
2008-7-31
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pubmed:abstractText |
Stem cell fate in the Arabidopsis thaliana shoot apical meristem (SAM) is controlled by WUSCHEL (WUS) and CLAVATA. Here, we examine BARD1 (for BRCA1-associated RING domain 1), which had previously been implicated in DNA repair functions; we find that it also regulates WUS expression. We observed severe SAM defects in the knockout mutant bard1-3. WUS transcripts accumulated >238-fold in bard1-3 compared with the wild type and were located mainly in the outermost cell layers instead of the usual organizing center. A specific WUS promoter region was recognized by nuclear protein extracts obtained from wild-type plants, and this protein-DNA complex was recognized by antibodies against BARD1. The double mutant (wus-1 bard1-3) showed prematurely terminated SAM structures identical to those of wus-1, indicating that BARD1 functions through regulation of WUS. BARD1 overexpression resulted in reduced WUS transcript levels, giving a wus-1-like phenotype. Either full-length BARD1 or a clone that encoded the C-terminal domain (BARD1:C-ter;bard1-3) was sufficient to complement the bard1-3 phenotype, indicating that BARD1 functions through its C-terminal domain. Our data suggest that BARD1 regulates SAM organization and maintenance by limiting WUS expression to the organizing center.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-10761929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-10915624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-11440721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-11440722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-11790560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-12000795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-12068101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-12070094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-12535342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-12582233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-14670373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-14826895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15004006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15133503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15208423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15343273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15367721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15375207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15501676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15563522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15833920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15917109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15952065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-15980263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16053711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16387832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16461579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16548064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16603652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16633366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16763149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16862119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-16957774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-17079273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-17429400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-17511964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-17766120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-19704708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-8565856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-8944023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-9160749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-9655815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18591352-9865698
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1482-93
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pubmed:dateRevised |
2010-9-22
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pubmed:meshHeading |
pubmed-meshheading:18591352-Arabidopsis,
pubmed-meshheading:18591352-Arabidopsis Proteins,
pubmed-meshheading:18591352-Blotting, Western,
pubmed-meshheading:18591352-Gene Expression Regulation, Plant,
pubmed-meshheading:18591352-Homeodomain Proteins,
pubmed-meshheading:18591352-In Situ Hybridization,
pubmed-meshheading:18591352-In Situ Nick-End Labeling,
pubmed-meshheading:18591352-Meristem,
pubmed-meshheading:18591352-Mutation,
pubmed-meshheading:18591352-Plants, Genetically Modified,
pubmed-meshheading:18591352-Polymerase Chain Reaction,
pubmed-meshheading:18591352-Promoter Regions, Genetic,
pubmed-meshheading:18591352-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
2008
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pubmed:articleTitle |
Mutation of Arabidopsis BARD1 causes meristem defects by failing to confine WUSCHEL expression to the organizing center.
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pubmed:affiliation |
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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