Linked Life Data

18571156

Source:http://linkedlifedata.com/resource/pubmed/id/18571156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2008-7-28
pubmed:abstractText
Cyclo-trans-4-L-hydroxyprolyl-L-serine (JBP485) is a dipeptide isolated from Laennec, and Laennec is a hydrolyzate of human placenta. Evidence has indicated that JBP485 exhibits potent anti-hepatitis activity. In this study, we investigated the protective effect and possible mechanisms of action of JBP485 in Concanavalin A (Con A)-induced hepatotoxicity in vitro. Two in vitro models were established. Model I: primary cultured female rat hepatocytes were only incubated with Con A (50 microg/ml); model II: co-culture system of hepatocytes and autologous splenic lymphocytes, both were stimulated with Con A (20 microg/ml). JBP485 (25 microM) was pre-incubated with the two models. Our results showed that JBP485 reduced cellular aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and tumor necrosis factor alpha (TNF-alpha) leakage following the application of Con A in both of the models. Potential protective mechanisms were elucidated by measuring DNA fragmentations, immunocytochemistry and RT-PCR. We showed that DNA fragmentations in hepatocytes were attenuated in the JBP485 pre-incubated groups, and at the same time, immunocytochemistry and RT-PCR indicated that expression levels of caspase-3 protein and mRNA in the JBP485 treated groups were decreased compared with those in the untreated groups. Moreover, intercellular adhesion molecule-1 (ICAM-1) was also down-regulated by this dipeptide. The results indicate that JBP485 exhibits hepatoprotective effect through inhibition of hepatocyte apoptosis and ICAM-1 expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
589
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-305
pubmed:meshHeading
pubmed-meshheading:18571156-Animals, pubmed-meshheading:18571156-Apoptosis, pubmed-meshheading:18571156-Aspartate Aminotransferases, pubmed-meshheading:18571156-Caspase 3, pubmed-meshheading:18571156-Cell Culture Techniques, pubmed-meshheading:18571156-Cells, Cultured, pubmed-meshheading:18571156-Coculture Techniques, pubmed-meshheading:18571156-Concanavalin A, pubmed-meshheading:18571156-Cytoprotection, pubmed-meshheading:18571156-DNA Fragmentation, pubmed-meshheading:18571156-Dose-Response Relationship, Drug, pubmed-meshheading:18571156-Female, pubmed-meshheading:18571156-Hepatocytes, pubmed-meshheading:18571156-Immunohistochemistry, pubmed-meshheading:18571156-Intercellular Adhesion Molecule-1, pubmed-meshheading:18571156-L-Lactate Dehydrogenase, pubmed-meshheading:18571156-Lymphocytes, pubmed-meshheading:18571156-Peptides, Cyclic, pubmed-meshheading:18571156-Protective Agents, pubmed-meshheading:18571156-RNA, Messenger, pubmed-meshheading:18571156-Rats, pubmed-meshheading:18571156-Rats, Wistar, pubmed-meshheading:18571156-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18571156-Tumor Necrosis Factor-alpha
pubmed:year
2008
pubmed:articleTitle
Protective effect of JBP485 on concanavalin A-induced hepatocyte toxicity in primary cultured rat hepatocytes.
pubmed:affiliation
Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Liaoning, Dalian, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't