Source:http://linkedlifedata.com/resource/pubmed/id/18563186
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
19
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| pubmed:dateCreated |
2008-9-16
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| pubmed:abstractText |
Impaired materno-placental perfusion causes two important obstetric complications, fetal growth restriction and preeclampsia. This study investigated whether adenoviral vector-mediated overexpression of vascular endothelial growth factor (VEGF) in the uterine arteries (UtAs) increases uterine artery blood flow (UBF). First-generation adenovirus vectors (5 x 10(11) particles) containing the VEGF gene (Ad.VEGF-A or -D) or the beta-galactosidase reporter gene (Ad.lacZ) were injected into the UtAs of pregnant sheep (n=6) at 88-102 days of gestation (term=145 days). UBF was measured using Doppler sonography before, and 4-7 days after injection. Mean UBF increased significantly from 233+/-156 (s.d.) ml min(-1) to 753+/-415 ml min(-1) following Ad.VEGF-A injection (P=0.005, n=5); Ad.lacZ infection had no significant effect. Organ bath experiments on uterine arterial sections 4-7 days after injection showed that, compared with Ad.lacZ vessels, Ad.VEGF-A-transduced vessels had a reduced contractile response to phenylephrine (E max 148+/-10.9 vs E max 228.2+/-27.5, P<0.05) but increased relaxation with bradykinin (pD2 (-log EC50) values 9.11+/-0.01 vs 8.65+/-0.11, P<0.05). Injection of Ad.VEGF-A into the UtAs increases UBF by enhancing vasodilatation. This may provide the basis for therapy in pregnancies complicated by uteroplacental insufficiency.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
1476-5462
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| pubmed:author |
pubmed-author:Abi-NaderKK,
pubmed-author:BoydMM,
pubmed-author:BuckleyS M KSM,
pubmed-author:CoonWW,
pubmed-author:DavidA LAL,
pubmed-author:MartinJJ,
pubmed-author:MehtaVV,
pubmed-author:NaderK AKA,
pubmed-author:PeeblesD MDM,
pubmed-author:RodeckC HCH,
pubmed-author:TorondelBB,
pubmed-author:WigleyVV,
pubmed-author:ZacharyII
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| pubmed:issnType |
Electronic
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| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1344-50
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| pubmed:dateRevised |
2008-12-30
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| pubmed:meshHeading |
pubmed-meshheading:18563186-Adenoviridae,
pubmed-meshheading:18563186-Animals,
pubmed-meshheading:18563186-Arteries,
pubmed-meshheading:18563186-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18563186-Female,
pubmed-meshheading:18563186-Fetal Growth Retardation,
pubmed-meshheading:18563186-Gene Expression,
pubmed-meshheading:18563186-Gene Therapy,
pubmed-meshheading:18563186-Genetic Vectors,
pubmed-meshheading:18563186-Injections, Intravenous,
pubmed-meshheading:18563186-Models, Animal,
pubmed-meshheading:18563186-Placental Circulation,
pubmed-meshheading:18563186-Pregnancy,
pubmed-meshheading:18563186-Regional Blood Flow,
pubmed-meshheading:18563186-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18563186-Sheep,
pubmed-meshheading:18563186-Transduction, Genetic,
pubmed-meshheading:18563186-Uterus,
pubmed-meshheading:18563186-Vascular Endothelial Growth Factor A,
pubmed-meshheading:18563186-Vasodilation
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| pubmed:year |
2008
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| pubmed:articleTitle |
Local delivery of VEGF adenovirus to the uterine artery increases vasorelaxation and uterine blood flow in the pregnant sheep.
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| pubmed:affiliation |
Prenatal Gene Therapy Group, Institute for Women's Health, Royal Free and University College London Medical School, London, UK. a.david@ucl.ac.uk
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|