Linked Life Data

18555842

Source:http://linkedlifedata.com/resource/pubmed/id/18555842

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-6-16
pubmed:abstractText
Peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) is considered to play a pivotal role in the exercise-induced metabolic adaptation of skeletal muscle. Although the oxidized form of nicotinamide adenine dinucloetide (NAD(+))-dependent histone deacetylase SIRT1 has been shown to mediate PGC-1alpha-induced metabolic adaptation, the effect of endurance exercise on the SIRT1 protein remains to be elucidated. The purposes of this study were (1) to investigate the distribution of SIRT1 and PGC-1alpha proteins in skeletal muscle and (2) to examine the effects of acute endurance exercise and low- or high-intensity exercise training on SIRT1 and PGC-1alpha protein expressions and on the metabolic components in rat skeletal muscle. Both the SIRT1 and PGC-1alpha proteins preferentially accumulate in red oxidative muscles. Acute endurance exercise on a motor-driven treadmill (20 m/min, 18.5% incline, 45 minutes) increases the PGC-1alpha protein expression at 18 hours after exercise and the SIRT1 protein expression at 2 hours after exercise in the soleus muscle. In the training experiment, the rats were divided into control, low-intensity (20 m/min, 18.5% incline, 90 min/d), and high-intensity (30 m/min, 18.5% incline, 60 min/d) training groups. After 14 days of training, the SIRT1 and PGC-1alpha proteins, hexokinase activity, mitochondrial proteins and enzyme activities, and glucose transporter 4 protein in the soleus muscle were increased by both trainings. In the plantaris muscle, SIRT1, hexokinase activity, mitochondrial proteins and enzyme activities, and glucose transporter 4 were increased by high-intensity training whereas the PGC-1alpha was not. These results suggest that endurance exercise increases the skeletal muscle SIRT1 protein content. In addition, the findings also raise the possibility that the SIRT1 protein expression may play a potentially important role in such adaptations, whereas an increase in the PGC-1alpha protein expression is not necessary for such adaptations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0026-0495
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
986-98
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:18555842-Animals, pubmed-meshheading:18555842-Blotting, Western, pubmed-meshheading:18555842-Body Weight, pubmed-meshheading:18555842-Caloric Restriction, pubmed-meshheading:18555842-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:18555842-Enzymes, pubmed-meshheading:18555842-Hexokinase, pubmed-meshheading:18555842-Male, pubmed-meshheading:18555842-Muscle, Skeletal, pubmed-meshheading:18555842-Muscle Proteins, pubmed-meshheading:18555842-Physical Conditioning, Animal, pubmed-meshheading:18555842-Physical Endurance, pubmed-meshheading:18555842-RNA-Binding Proteins, pubmed-meshheading:18555842-Rats, pubmed-meshheading:18555842-Rats, Wistar, pubmed-meshheading:18555842-Sirtuin 1, pubmed-meshheading:18555842-Sirtuins, pubmed-meshheading:18555842-Transcription Factors, pubmed-meshheading:18555842-Weight Loss
pubmed:year
2008
pubmed:articleTitle
Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor gamma coactivator-1alpha protein expressions in rat skeletal muscle.
pubmed:affiliation
Institute of Health Science, Kyushu University, Kasuga, Fukuoka 816-8580, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't