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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1991-8-27
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pubmed:abstractText |
The transbilayer cholesterol distribution of human erythrocytes was examined by two independent techniques, quenching of dehydroergosterol fluorescence and fluorescence photobleaching of NBD-cholesterol. Dehydroergosterol in conjunction with leaflet selective quenching showed that, at equilibrium, 75% of the sterol was localized to the inner leaflet of resealed erythrocyte ghosts. NBD-cholesterol and fluorescence photobleaching displayed two diffusion values in both resealed ghosts and intact erythrocytes. The fractional contribution of the fast and slow diffusion constants of NBD-labelled cholesterol represent its inner and outer leaflet distribution. At room temperature the plasma membrane inner leaflet of erythrocyte ghosts as well as intact erythrocytes cells contained 78% of the plasma membrane sterol. The erythrocyte membrane transbilayer distribution of sterol was independent of temperature. In conclusion, dehydroergosterol and NBD-cholesterol data are consistent with an enrichment of cholesterol in the inner leaflet of the human erythrocyte.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Diphenylhexatriene,
http://linkedlifedata.com/resource/pubmed/chemical/Ergosterol,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A,
http://linkedlifedata.com/resource/pubmed/chemical/Trinitrobenzenesulfonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/dehydroergosterol
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
1066
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-92
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1854783-Cholesterol,
pubmed-meshheading:1854783-Diphenylhexatriene,
pubmed-meshheading:1854783-Ergosterol,
pubmed-meshheading:1854783-Erythrocyte Membrane,
pubmed-meshheading:1854783-Erythrocytes,
pubmed-meshheading:1854783-Fluorescence,
pubmed-meshheading:1854783-Humans,
pubmed-meshheading:1854783-Kinetics,
pubmed-meshheading:1854783-Lipid Bilayers,
pubmed-meshheading:1854783-Liposomes,
pubmed-meshheading:1854783-Phosphatidylethanolamines,
pubmed-meshheading:1854783-Phospholipases A,
pubmed-meshheading:1854783-Trinitrobenzenesulfonic Acid
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pubmed:year |
1991
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pubmed:articleTitle |
Transmembrane distribution of sterol in the human erythrocyte.
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pubmed:affiliation |
Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, OH.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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