Source:http://linkedlifedata.com/resource/pubmed/id/18502582
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2008-9-22
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| pubmed:abstractText |
It has been postulated that the G protein-coupled receptor, GPR55, is a third cannabinoid receptor. Given that the ligands at the CB(1) and CB(2) receptors are effective analgesic and anti-inflammatory agents, the role of GPR55 in hyperalgesia associated with inflammatory and neuropathic pain has been investigated. As there are no well-validated GPR55 tool compounds, a GPR55 knockout (GPR55(-/-)) mouse line was generated and fully backcrossed onto the C57BL/6 strain. General phenotypic analysis of GPR55(-/-) mice revealed no obvious primary differences, compared with wild-type (GPR55(+/+)) littermates. GPR55(-/-) mice were then tested in the models of adjuvant-induced inflammation and partial nerve ligation. Following intraplantar administration of Freund's complete adjuvant (FCA), inflammatory mechanical hyperalgesia was completely absent in GPR55(-/-) mice up to 14 days post-injection. Cytokine profiling experiments showed that at 14 days post-FCA injection there were increased levels of IL-4, IL-10, IFN gamma and GM-CSF in paws from the FCA-injected GPR55(-/-) mice when compared with the FCA-injected GPR55(+/+) mice. This suggests that GPR55 signalling can influence the regulation of certain cytokines and this may contribute to the lack of inflammatory mechanical hyperalgesia in the GPR55(-/-) mice. In the model of neuropathic hypersensitivity, GPR55(-/-) mice also failed to develop mechanical hyperalgesia up to 28 days post-ligation. These data clearly suggest that the manipulation of GPR55 may have therapeutic potential in the treatment of both inflammatory and neuropathic pain.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1872-6623
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| pubmed:author |
pubmed-author:BaileyLeigh TLT,
pubmed-author:BillintonAndyA,
pubmed-author:BrownAndrew JAJ,
pubmed-author:ChessellIain PIP,
pubmed-author:ChongElizabethE,
pubmed-author:FulleyloveMichaelM,
pubmed-author:GreenPaula JPJ,
pubmed-author:HatcherJon PJP,
pubmed-author:HughesJane PJP,
pubmed-author:LancasterHilary CHC,
pubmed-author:ManderPalwinder KPK,
pubmed-author:MorrisonAlastair DAD,
pubmed-author:RichardsonJill CJC,
pubmed-author:ShaplandEllen MEM,
pubmed-author:SmithJason CJC,
pubmed-author:StatonPenny CPC,
pubmed-author:WalkerDeborah JDJ,
pubmed-author:WiseAlanA
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
30
|
| pubmed:volume |
139
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
225-36
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| pubmed:meshHeading |
pubmed-meshheading:18502582-Amino Acid Sequence,
pubmed-meshheading:18502582-Animals,
pubmed-meshheading:18502582-Base Sequence,
pubmed-meshheading:18502582-Female,
pubmed-meshheading:18502582-Hyperalgesia,
pubmed-meshheading:18502582-Inflammation,
pubmed-meshheading:18502582-Inflammation Mediators,
pubmed-meshheading:18502582-Ligation,
pubmed-meshheading:18502582-Male,
pubmed-meshheading:18502582-Mice,
pubmed-meshheading:18502582-Mice, Inbred C57BL,
pubmed-meshheading:18502582-Mice, Knockout,
pubmed-meshheading:18502582-Molecular Sequence Data,
pubmed-meshheading:18502582-Neuralgia,
pubmed-meshheading:18502582-Pain Measurement,
pubmed-meshheading:18502582-Physical Stimulation,
pubmed-meshheading:18502582-Receptors, Cannabinoid
|
| pubmed:year |
2008
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| pubmed:articleTitle |
The putative cannabinoid receptor GPR55 plays a role in mechanical hyperalgesia associated with inflammatory and neuropathic pain.
|
| pubmed:affiliation |
Neurology Centre of Excellence for Drug Discovery, GlaxoSmithKline, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK. Penny.C.Staton@gsk.com
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| pubmed:publicationType |
Journal Article,
Comparative Study
|