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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2008-6-24
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pubmed:abstractText |
Interleukin-1 receptor antagonist (IL-1Ra), is a natural blocker of the inflammatory cytokine interleukin-1. Using a rat adjuvant-induced arthritis (AIA) model of rheumatoid arthritis (RA), we examined the protective effects of IL-1Ra in bone metabolism in vivo after folate-mediated nonviral gene delivery. We detected secreted human IL-1Ra protein in serum and cultured primary osteoblasts of rats that were treated with chitosan-IL-1Ra and folate-IL-1Ra-chitosan nanoparticles, respectively. In vivo, IL-1Ra gene delivery significantly reverted alterations in bone turnover observed in arthritic animals by modulating the level of osteocalcin (OC) as well as the activities of alkaline phosphatase and tartrate-resistant acid phosphatase. The protective effects of these nanoparticles were evident from the decrease in the expression levels of interleukine-1beta and prostaglandin E(2) as well as osteoclast number and other histopathological findings. Compared to naked DNA and chitosan-DNA, folate-chitosan-DNA nanoparticles were less cytotoxic and enhanced IL-1Ra protein synthesis in vitro and offered a better protection against inflammation and abnormal bone metabolism in vivo. Nonviral gene therapy with folate-chitosan-DNA nanoparticles containing the IL-1 Ra gene seemed to protect against bone damage and inflammation in rat adjuvant-induced arthritis model.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acid Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Chitosan,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Folic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin 1 Receptor Antagonist...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/tartrate-resistant acid phosphatase
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1525-0024
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1243-51
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pubmed:meshHeading |
pubmed-meshheading:18500247-Acid Phosphatase,
pubmed-meshheading:18500247-Alkaline Phosphatase,
pubmed-meshheading:18500247-Animals,
pubmed-meshheading:18500247-Arthritis, Experimental,
pubmed-meshheading:18500247-Bone and Bones,
pubmed-meshheading:18500247-Chitosan,
pubmed-meshheading:18500247-DNA,
pubmed-meshheading:18500247-Dinoprostone,
pubmed-meshheading:18500247-Folic Acid,
pubmed-meshheading:18500247-Gene Therapy,
pubmed-meshheading:18500247-Gene Transfer Techniques,
pubmed-meshheading:18500247-Humans,
pubmed-meshheading:18500247-Interleukin 1 Receptor Antagonist Protein,
pubmed-meshheading:18500247-Interleukin-1beta,
pubmed-meshheading:18500247-Isoenzymes,
pubmed-meshheading:18500247-Nanoparticles,
pubmed-meshheading:18500247-Osteoblasts,
pubmed-meshheading:18500247-Rats,
pubmed-meshheading:18500247-Tumor Necrosis Factor-alpha
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pubmed:year |
2008
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pubmed:articleTitle |
Bone-protective effects of nonviral gene therapy with folate-chitosan DNA nanoparticle containing interleukin-1 receptor antagonist gene in rats with adjuvant-induced arthritis.
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pubmed:affiliation |
Orthopaedics Research Laboratory, Research Center, Sacré-Coeur Hospital, University of Montreal, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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