Linked Life Data

18475304

Source:http://linkedlifedata.com/resource/pubmed/id/18475304

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2008-5-28
pubmed:abstractText
Bystander effects induced by cytoplasmic irradiation have been reported recently. However, the mechanism(s) underlying, such as the functional role of mitochondria, is not clear. In the present study, we used either mtDNA-depleted (rho(0)) A(L) or normal (rho(+)) A(L) cells as irradiated donor cells and normal human skin fibroblasts as receptor cells in a series of medium transfer experiments to investigate the mitochondria-related signal process. Our results indicated that mtDNA-depleted cells or normal A(L) cells treated with mitochondrial respiratory chain function inhibitors had an attenuated gamma-H2AX induction, which indicates that mitochondria play a functional role in bystander effects. Moreover, it was found that treatment of normal A(L) donor cells with specific inhibitors of NOS, or inhibitor of mitochondrial calcium uptake (ruthenium red) significantly decreased gamma-H2AX induction and that radiation could stimulate cellular NO and O(2)(*-) production in irradiated rho(+) A(L) cells, but not in rho(0) A(L) cells. These observations, together with the findings that ruthenium red treatment significantly reduced the NO and O(2)(*-) levels in irradiated rho(+) A(L) cells, suggest that radiation-induced NO derived from mitochondria might be an intracellular bystander factor and calcium-dependent mitochondrial NOS might play an essential role in the process.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1532-1827
pubmed:author
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1839-44
pubmed:dateRevised
2009-9-11
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Mitochondria-dependent signalling pathway are involved in the early process of radiation-induced bystander effects.
pubmed:affiliation
Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural