rdf:type |
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0011065,
umls-concept:C0012854,
umls-concept:C0017337,
umls-concept:C0036126,
umls-concept:C0080194,
umls-concept:C0085732,
umls-concept:C0243125,
umls-concept:C0348011,
umls-concept:C0392747,
umls-concept:C0699900,
umls-concept:C1274040,
umls-concept:C1554963,
umls-concept:C1705822,
umls-concept:C1709854
|
pubmed:issue |
3
|
pubmed:dateCreated |
1991-3-11
|
pubmed:abstractText |
The genes encoding the restriction-modification system StyLTI of Salmonella typhimurium were inserted in vivo into the conjugative plasmid pULB21. This allowed us to transfer the StyLTI genes at a very high frequency and to monitor the fate of recipient cells after mating. Transfer of the StyLTI restriction and modification genes into a modificationless recipient was lethal and resulted in degradation of the cell's DNA. This indicates that, in contrast to any other known restriction-modification systems, StyLTI cannot be established after horizontal transfer into a naive host.
|
pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0021-9193
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
173
|
pubmed:geneSymbol |
mod,
res
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1328-30
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:1846862-Conjugation, Genetic,
pubmed-meshheading:1846862-DNA, Bacterial,
pubmed-meshheading:1846862-DNA Modification Methylases,
pubmed-meshheading:1846862-DNA Restriction Enzymes,
pubmed-meshheading:1846862-Deoxyribonucleases, Type III Site-Specific,
pubmed-meshheading:1846862-Genes, Bacterial,
pubmed-meshheading:1846862-Kinetics,
pubmed-meshheading:1846862-Salmonella typhimurium,
pubmed-meshheading:1846862-Site-Specific DNA-Methyltransferase (Adenine-Specific)
|
pubmed:year |
1991
|
pubmed:articleTitle |
Transfer of the genes for the StyLTI restriction-modification system of Salmonella typhimurium to strains lacking modification ability results in death of the recipient cells and degradation of their DNA.
|
pubmed:affiliation |
Département de Biologie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
|
pubmed:publicationType |
Journal Article
|