| pubmed-article:18455878 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18455878 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:18455878 | lifeskim:mentions | umls-concept:C0079883 | lld:lifeskim |
| pubmed-article:18455878 | lifeskim:mentions | umls-concept:C1708517 | lld:lifeskim |
| pubmed-article:18455878 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18455878 | pubmed:dateCreated | 2008-5-27 | lld:pubmed |
| pubmed-article:18455878 | pubmed:abstractText | The subplate plays an important role in forming neuronal connections during early cortical development. We characterized by the use of whole-cell and cell-attached patch-clamp recordings in coronal brain slices from newborn mice (postnatal day [P] 0-3) the functional properties of two major pathways onto subplate neurons (SPn), the thalamocortical and the intra-subplate synaptic input. The two afferent pathways were stimulated extracellularly with bipolar electrodes placed in the thalamus and the subplate, respectively. Synaptically evoked and pharmacologically isolated N-methyl-d-aspartate receptor (NMDAR) -mediated responses with an onset latency of approximately 6 ms could be reliably recorded in P0-3 SPn. Whereas the intra-subplate input revealed a pronounced facilitation using paired pulse stimulation at 60-120 ms or repetitive activation at 10-40 Hz, the thalamocortical input was either stable or markedly suppressed under these conditions. Single cell reverse transcription PCR revealed the expression of the NR2A, B and D subunit in all investigated SPn. The intra-subplate and the thalamocortical synaptic input did not differ in their sensitivity to NVP-AAM077 or ifenprodil, indicating that both synaptic inputs have a similar NR2A/2B subunit composition. At P0, NMDAR-mediated synaptic inputs arising from the thalamus were significantly larger as compared with the intra-subplate input. This difference could no longer be detected in P2-3 SPn, indicating an input-specific developmental regulation during the first Ps. Our data indicate that the thalamocortical and intra-subplate synaptic input onto P0-3 SPn differs in functional, molecular and developmental properties. The intra-subplate synaptic input shows more mature functional properties and sustains high stimulation frequencies, thereby promoting the immature thalamocortical input to the developing neocortical circuit. | lld:pubmed |
| pubmed-article:18455878 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18455878 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18455878 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18455878 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18455878 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18455878 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18455878 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18455878 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18455878 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18455878 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:18455878 | pubmed:issn | 0306-4522 | lld:pubmed |
| pubmed-article:18455878 | pubmed:author | pubmed-author:HirschSS | lld:pubmed |
| pubmed-article:18455878 | pubmed:author | pubmed-author:LuhmannH JHJ | lld:pubmed |
| pubmed-article:18455878 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18455878 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:18455878 | pubmed:volume | 153 | lld:pubmed |
| pubmed-article:18455878 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18455878 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18455878 | pubmed:pagination | 1092-102 | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:meshHeading | pubmed-meshheading:18455878... | lld:pubmed |
| pubmed-article:18455878 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18455878 | pubmed:articleTitle | Pathway-specificity in N-methyl-D-aspartate receptor-mediated synaptic inputs onto subplate neurons. | lld:pubmed |
| pubmed-article:18455878 | pubmed:affiliation | Institute of Physiology and Pathophysiology, University of Mainz, Duesbergweg 6, Mainz, Germany. | lld:pubmed |
| pubmed-article:18455878 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18455878 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:18455878 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18455878 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18455878 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18455878 | lld:pubmed |
