Linked Life Data

18455878

Source:http://linkedlifedata.com/resource/pubmed/id/18455878

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-5-27
pubmed:abstractText
The subplate plays an important role in forming neuronal connections during early cortical development. We characterized by the use of whole-cell and cell-attached patch-clamp recordings in coronal brain slices from newborn mice (postnatal day [P] 0-3) the functional properties of two major pathways onto subplate neurons (SPn), the thalamocortical and the intra-subplate synaptic input. The two afferent pathways were stimulated extracellularly with bipolar electrodes placed in the thalamus and the subplate, respectively. Synaptically evoked and pharmacologically isolated N-methyl-d-aspartate receptor (NMDAR) -mediated responses with an onset latency of approximately 6 ms could be reliably recorded in P0-3 SPn. Whereas the intra-subplate input revealed a pronounced facilitation using paired pulse stimulation at 60-120 ms or repetitive activation at 10-40 Hz, the thalamocortical input was either stable or markedly suppressed under these conditions. Single cell reverse transcription PCR revealed the expression of the NR2A, B and D subunit in all investigated SPn. The intra-subplate and the thalamocortical synaptic input did not differ in their sensitivity to NVP-AAM077 or ifenprodil, indicating that both synaptic inputs have a similar NR2A/2B subunit composition. At P0, NMDAR-mediated synaptic inputs arising from the thalamus were significantly larger as compared with the intra-subplate input. This difference could no longer be detected in P2-3 SPn, indicating an input-specific developmental regulation during the first Ps. Our data indicate that the thalamocortical and intra-subplate synaptic input onto P0-3 SPn differs in functional, molecular and developmental properties. The intra-subplate synaptic input shows more mature functional properties and sustains high stimulation frequencies, thereby promoting the immature thalamocortical input to the developing neocortical circuit.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1092-102
pubmed:meshHeading
pubmed-meshheading:18455878-6-Cyano-7-nitroquinoxaline-2,3-dione, pubmed-meshheading:18455878-Age Factors, pubmed-meshheading:18455878-Animals, pubmed-meshheading:18455878-Animals, Newborn, pubmed-meshheading:18455878-Cerebral Cortex, pubmed-meshheading:18455878-Dose-Response Relationship, Radiation, pubmed-meshheading:18455878-Electric Stimulation, pubmed-meshheading:18455878-Excitatory Amino Acid Antagonists, pubmed-meshheading:18455878-Excitatory Postsynaptic Potentials, pubmed-meshheading:18455878-Mice, pubmed-meshheading:18455878-Neural Pathways, pubmed-meshheading:18455878-Neurons, pubmed-meshheading:18455878-Patch-Clamp Techniques, pubmed-meshheading:18455878-Piperazines, pubmed-meshheading:18455878-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:18455878-Synapses, pubmed-meshheading:18455878-Thalamus
pubmed:year
2008
pubmed:articleTitle
Pathway-specificity in N-methyl-D-aspartate receptor-mediated synaptic inputs onto subplate neurons.
pubmed:affiliation
Institute of Physiology and Pathophysiology, University of Mainz, Duesbergweg 6, Mainz, Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't