Linked Life Data

18453132

Source:http://linkedlifedata.com/resource/pubmed/id/18453132

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-5-5
pubmed:abstractText
Polymorphonuclear neutrophils (PMN) represent the dominant cell in the acute response to microbial infection. Effective antimicrobial activity reflects the combined action of soluble agents in plasma with PMN-derived reactive oxygen species and granule proteins, including the azurophilic granule protein myeloperoxidase (MPO). The inhibition or absence of the MPO-H2O2-halide system results in marked reduction in PMN killing of a variety of microbes, implicating its relative prominence in the hierarchy of PMN antimicrobial systems. Although the most profound clinical defects are manifested in patients lacking the capacity to generate reactive oxygen species, as seen in chronic granulomatous disease, an inherited deficiency of MPO can also increase the frequency or severity of clinical infections. As a first step in evaluating the MPO-dependent system, determination of peroxidase activity of isolated leukocytes enriched for PMN coupled with histochemical staining of leukocytes allows assessment of functional MPO within PMN. Immunoblotting of the isolated leukocytes for MPO provides additional information, supplying insight into the molecular basis of the observed absence of functional enzyme.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
412
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
525-30
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Diagnostic assays for myeloperoxidase deficiency.
pubmed:affiliation
Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Affairs Medical Center, Coralville, Iowa City, IA, USA.
pubmed:publicationType
Journal Article