Source:http://linkedlifedata.com/resource/pubmed/id/18448359
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2008-6-13
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| pubmed:databankReference | |
| pubmed:abstractText |
Double-stranded RNA-activated protein kinase (PKR) plays an important role in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In the present study, a PKR-like gene was cloned and characterized from rare minnow Gobiocypris rarus. The full length of the rare minnow PKR-like (GrPKZ) cDNA is 1946 bp in length and encodes a polypeptide of 503 amino acids with an estimated molecular mass of 57,355 Da and a predicted isoelectric point of 5.83. Analysis of the deduced amino acid sequence indicated that the mature peptide contains two Zalpha domains and one S_TKc domain, and is most similar to the crucian carp (Carassius auratus) PKR-like amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed that GrPKZ mRNA expression is at low levels in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus, GrPKZ expression was up-regulated from 24h post-injection and lasted until the fish became moribund (P<0.05). Following infection with Aeromonas hydrophila, GrPKZ transcripts were induced at 24h post-injection (P<0.05) and returned to control levels at 120 h post-injection. These data imply that GrPKZ is involved in antiviral defense and Toll-like receptor 4 signaling pathway in bacterial infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
1050-4648
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
25
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
106-13
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:18448359-Aeromonas hydrophila,
pubmed-meshheading:18448359-Amino Acid Sequence,
pubmed-meshheading:18448359-Animals,
pubmed-meshheading:18448359-Base Sequence,
pubmed-meshheading:18448359-Cloning, Molecular,
pubmed-meshheading:18448359-Cyprinidae,
pubmed-meshheading:18448359-Gene Expression Profiling,
pubmed-meshheading:18448359-Gene Expression Regulation,
pubmed-meshheading:18448359-Gram-Negative Bacterial Infections,
pubmed-meshheading:18448359-Molecular Sequence Data,
pubmed-meshheading:18448359-Phylogeny,
pubmed-meshheading:18448359-RNA, Messenger,
pubmed-meshheading:18448359-Reoviridae,
pubmed-meshheading:18448359-Reoviridae Infections,
pubmed-meshheading:18448359-Sequence Alignment,
pubmed-meshheading:18448359-Time Factors,
pubmed-meshheading:18448359-eIF-2 Kinase
|
| pubmed:year |
2008
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| pubmed:articleTitle |
Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus.
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| pubmed:affiliation |
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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