Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-4-25
pubmed:abstractText
Protein oligomerization serves an important function in biological processes, yet solving structures of protein oligomers has always been a challenge. For solution NMR, the challenge arises both from the increased size of these systems and, in the case of homo-oligomers, from ambiguities in assignment of intra- as opposed to intersubunit NOEs. In this study, we present a residual dipolar coupling (RDC)-assisted method for constructing models of homo-oligomers with purely rotational symmetry. Utilizing the fact that one of the principal axes of the tensor describing the alignment needed for RDC measurement is always parallel to the oligomer symmetry axis, it is possible to greatly restrict possible models for the oligomer. Here, it is shown that, if the monomer structure is known, all allowed dimer models can be constructed using a grid search algorithm and evaluated based on RDC simulations and the quality of the interface between the subunits. Using the Bacillus subtilis protein YkuJ as an example, it is shown that the evaluation criteria based on just two sets of NH RDCs are very selective and can unambiguously produce a model in good agreement with an existing X-ray structure of YkuJ.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-10341140, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-10617446, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-10940245, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-11456575, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-11734640, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-12565051, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-12912836, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15014231, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15040978, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15211503, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15491616, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15491619, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-1549581, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15710392, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15808221, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-15993087, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-16140525, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-16154092, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-16222654, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-16450362, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-16897780, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-16927360, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-18026911, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-7616572, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-8107108, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-8263940, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-8552589, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-8720829, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-8744570, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-9353189, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-9571116, http://linkedlifedata.com/resource/pubmed/commentcorrection/18436958-9846877
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1469-896X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
899-907
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
RDC-assisted modeling of symmetric protein homo-oligomers.
pubmed:affiliation
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies, Research Support, N.I.H., Extramural