Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
Cytochrome P450nor (P450nor) is a nitric oxide (NO) reductase involved in fungal denitrification. Denitrification is a biological process in which nitrate or nitrite is reduced to gaseous nitrogen, the reverse reaction of nitrogen fixation. It therefore plays an important role in maintaining global environmental homeostasis. The involvement of P450nor in fungal denitrification indicates that denitrification not only occurs in prokaryotic bacteria, but also in eukaryotic fungi. In addition, the reduction of NO to nitrous oxide catalyzed by P450nor has added new insight into the function of cytochrome P450s, which are usually monooxygenases. Currently, five isozymes of P450nor have been isolated from the subdivisions of eumycota, and studies on the function and structure of P450nor have provided important information for both molecular mechanisms of P450 reactions and wastewater treatment. This chapter describes the screening of NO reductase activities, cloning, purification, and functional analysis of P450nor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
437
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-33
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor.
pubmed:affiliation
Department of Biology, University of Kentucky, Lexington, Kentucky, USA.
pubmed:publicationType
Journal Article, Review