Linked Life Data

18429284

Source:http://linkedlifedata.com/resource/pubmed/id/18429284

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-22
pubmed:abstractText
With the introduction of DNA microarrays as novel analytical tools, the determination of thousands of binding events in one reaction became possible. The developed technology platforms are not limited to nucleic acids, and, in principle, every ligand-binding assay that works on solid phase can be miniaturized and brought into an array format. This unit explains how protein microarrays can be generated using equipment originally designed for DNA microarrays and how multiplexed assays for the quantification of proteins are set up. A protocol that describes a parallelized system for detecting autoantibodies in human serum is included as an example, and it is shown how existing sandwich immunoassays can be miniaturized and performed in array format. The unit also provides some theoretical background and commentary on the problems associated with this still-novel technology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1934-3663
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 23.5
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Protein microarrays.
pubmed:affiliation
NMI-Natural and Medical Science Institute at the University of Tübingen, Tübingen, Germany.
pubmed:publicationType
Journal Article