Linked Life Data

18412541

Source:http://linkedlifedata.com/resource/pubmed/id/18412541

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2009-1-2
pubmed:abstractText
The gene encoding Psp HJ147 UDG (Psychrobacter sp. HJ147 uracil-DNA glycosylase) was cloned and sequenced. The gene consists of 735 bp for coding a protein with 244 amino acid residues. The deduced amino acid sequence of Psp HJ147 UDG showed a high similarity to that of Psychrobacter articus, Psychrobacter cryohalolentis K5 and Psychrobacter sp. PRwf-1. The PCR-amplified Psp HJ147 UDG gene was expressed under the control of the T7lac promoter on pTYB1 in Escherichia coli BL21(DE3). The expressed enzyme was purified with IMPACT-CN (intein-mediated purification with an affinity chitin-binding tag) system. The optimum pH and temperature of the purified enzyme were 7.0-7.5 and 20-25 degrees C respectively. The optimum NaCl and KCl concentrations for the activity of the purified enzyme ranged from 50 to75 mM. The half-life of the enzyme at 50 degrees C was approx. 45 s. These heat-labile characteristics enabled Psp HJ147 UDG to control carry-over contamination in direct PCR without loss of the PCR product. Psp HJ147 UDG's contaminant control in both direct PCR and indirect PCR exhibited superiority over the UDG of the marine psychrophilic bacterium strain BMTU 3346 and that of E. coli.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1470-8744
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-75
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Characterization of heat-labile uracil-DNA glycosylase from Psychrobacter sp. HJ147 and its application to the polymerase chain reaction.
pubmed:affiliation
Department of Genetic Engineering, Sungkyunkwan University, 300 Chunchun-dong, Jangan-gu, Suwon 440-746, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't