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pubmed:abstractText |
We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.
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pubmed:affiliation |
Department of Chemical and Systems Biology, Bio-X Program, Clark Center, 318 Campus Drive, Stanford University School of Medicine, Stanford, California 94305, USA. guignet@stanford.edu
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