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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2008-4-23
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pubmed:abstractText |
In this study, 2-Cys Plasmodium berghei ANKA (PbA) peroxiredoxin (Prx) was identified as an antigenic protein recognized by an anti-PbA IgE antibody using two-dimensional polyacrylamide gel electrophoresis and proteomic analysis. Innate immune responses to PbAPrx were examined using cells from mice deficient in Toll-like receptors (TLR) or related molecules, and it was demonstrated that responses were severely impaired in TLR4(-/-), MyD88(-/-) and MD-2(-/-) mice, but not in Toll/IL-1 receptor domain-containing adaptor inducing IFN-gamma (TRIF)(-/-), TLR2(-/-) or radioprotective 105 (RP105)(-/-) mice. An association between PbAPrx and TLR4 was observed following immunoprecipitation and immunoblotting, suggesting that PbAPrx was associated with TLR4/MD-2. Interactions between Prx and TLR4/MD-2 were also examined by flow cytometry using TLR4/MD-2- or TLR2-expressing cells. NFkappaB/GFP activity was observed in TLR4/MD-2- but not in TLR2-expressing cells following stimulation with Prx. However, this effect was not observed after treatment with proteinase K, suggesting that PbAPrx is a protein ligand for TLR4 and that the PbAPrx activity observed in this study is not due to contamination with LPS. These findings indicate that malarial Prx induces IgE-mediated protection through FcepsilonRI on mast cells and innate immunity through TLR4 with MyD88 and MD-2, suggesting a novel function for malarial Prx in innate and acquired immune responses in malaria.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Cys peroxiredoxin, Plasmodium...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Ly96 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Antigen 96,
http://linkedlifedata.com/resource/pubmed/chemical/Myd88 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myeloid Differentiation Factor 88,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxiredoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgE
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1341-50
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pubmed:meshHeading |
pubmed-meshheading:18398934-Animals,
pubmed-meshheading:18398934-Antibodies, Anti-Idiotypic,
pubmed-meshheading:18398934-Antigens, CD14,
pubmed-meshheading:18398934-B-Lymphocytes,
pubmed-meshheading:18398934-Cell Line,
pubmed-meshheading:18398934-Cytokines,
pubmed-meshheading:18398934-Dendritic Cells,
pubmed-meshheading:18398934-Female,
pubmed-meshheading:18398934-Immunoglobulin E,
pubmed-meshheading:18398934-Lymphocyte Antigen 96,
pubmed-meshheading:18398934-Macrophages, Peritoneal,
pubmed-meshheading:18398934-Malaria,
pubmed-meshheading:18398934-Male,
pubmed-meshheading:18398934-Mast Cells,
pubmed-meshheading:18398934-Mice,
pubmed-meshheading:18398934-Mice, Inbred C57BL,
pubmed-meshheading:18398934-Mice, Knockout,
pubmed-meshheading:18398934-Myeloid Differentiation Factor 88,
pubmed-meshheading:18398934-NF-kappa B,
pubmed-meshheading:18398934-Peroxiredoxins,
pubmed-meshheading:18398934-Plasmodium berghei,
pubmed-meshheading:18398934-Protein Binding,
pubmed-meshheading:18398934-Toll-Like Receptor 4,
pubmed-meshheading:18398934-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
Mast cell-mediated immune responses through IgE antibody and Toll-like receptor 4 by malarial peroxiredoxin.
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pubmed:affiliation |
Division of Infectious Genetics, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan. furuta@ims.u-tokyo.ac.jp
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pubmed:publicationType |
Journal Article
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