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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1992-4-29
|
| pubmed:abstractText |
A chemically synthesized alpha-hANP gene was inserted into plasmid YFD18, which was an expression-secretion vector of yeast. The recombinant then transformed in the yeast Y33. The expression level of yeast transformants was about 700 micrograms ANP/L detected by RIA. More than 99% of expression products were secreted in the culture medium. N-terminal analysis of purified product showed that the first 4 amino acid residues of alpha-hANP were deleted.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1001-652X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
34
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1478-84
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:1839353-Amino Acid Sequence,
pubmed-meshheading:1839353-Atrial Natriuretic Factor,
pubmed-meshheading:1839353-Cloning, Molecular,
pubmed-meshheading:1839353-Escherichia coli,
pubmed-meshheading:1839353-Genes,
pubmed-meshheading:1839353-Molecular Sequence Data,
pubmed-meshheading:1839353-Plasmids,
pubmed-meshheading:1839353-Recombination, Genetic,
pubmed-meshheading:1839353-Saccharomyces cerevisiae
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Synthesis and secretion of human atrial natriuretic peptide in Saccharomyces cerevisiae.
|
| pubmed:affiliation |
Institute of Genetics, Fudan University, Shanghai, PRC.
|
| pubmed:publicationType |
Journal Article
|