Source:http://linkedlifedata.com/resource/pubmed/id/18371438
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2008-3-28
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| pubmed:abstractText |
Mammalian spermatogenesis is maintained by stem cell capacity within undifferentiated spermatogonial subpopulation. Here, using a combination of surface markers, we describe a purification method for undifferentiated spermatogonia. Flow cytometric analysis revealed that this population is composed of Plzf-positive cells and exhibits quiescence and the side population phenotype, fulfilling general stem cell criteria. We then applied this method to analyze undifferentiated spermatogonia and stem cell activity of Atm(-/-) mice. Atm(-/-) testis shows progressive depletion of undifferentiated spermatogonia accompanied by cell-cycle arrest. In Atm(-/-) undifferentiated spermatogonia, a self-renewal defect was observed in vitro and in vivo. Accumulation of DNA damage and activation of the p19(Arf)-p53-p21(Cip1/Waf1) pathway were observed in Atm(-/-) undifferentiated spermatogonia. Moreover, suppression of p21(Cip1/Waf1) in an Atm(-/-) background restored transplantation ability of undifferentiated spermatogonia, indicating that ATM plays an essential role in maintenance of undifferentiated spermatogonia and their stem cell capacity by suppressing DNA damage-induced cell-cycle arrest.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ataxia telangiectasia mutated...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
|
| pubmed:issn |
1875-9777
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
7
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| pubmed:volume |
2
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
170-82
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| pubmed:dateRevised |
2011-11-2
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| pubmed:meshHeading |
pubmed-meshheading:18371438-Animals,
pubmed-meshheading:18371438-Cell Cycle,
pubmed-meshheading:18371438-Cell Cycle Proteins,
pubmed-meshheading:18371438-Cell Differentiation,
pubmed-meshheading:18371438-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:18371438-DNA Damage,
pubmed-meshheading:18371438-DNA-Binding Proteins,
pubmed-meshheading:18371438-Flow Cytometry,
pubmed-meshheading:18371438-Immunohistochemistry,
pubmed-meshheading:18371438-Male,
pubmed-meshheading:18371438-Mice,
pubmed-meshheading:18371438-Mice, Knockout,
pubmed-meshheading:18371438-Protein-Serine-Threonine Kinases,
pubmed-meshheading:18371438-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18371438-Signal Transduction,
pubmed-meshheading:18371438-Spermatogenesis,
pubmed-meshheading:18371438-Spermatogonia,
pubmed-meshheading:18371438-Stem Cells,
pubmed-meshheading:18371438-Tumor Suppressor Proteins
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| pubmed:year |
2008
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| pubmed:articleTitle |
Stem cell defects in ATM-deficient undifferentiated spermatogonia through DNA damage-induced cell-cycle arrest.
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| pubmed:affiliation |
Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582, Japan. keiyot@gmail.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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