Linked Life Data

18363794

Source:http://linkedlifedata.com/resource/pubmed/id/18363794

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-4-23
pubmed:abstractText
The co-ordination and synchronization of DNA replication, chromosome partitioning and cell division in bacteria are critical to survival. In Escherichia coli, the septal protein FtsK links cell division and chromosome segregation through its integral membrane N-terminal and cytoplasmic C-terminal domains. FtsK is responsible for promoting decatenation and dimer resolution in the later stages of chromosome segregation by activating recombination at dif by the site-specific Xer recombinases. Here, we formally demonstrate, using novel assay based on real-time quantitative polymerase chain reaction, that dif recombination depends not only on proteins upstream of FtsK in the septum assembly pathway, but also on the activity of downstream proteins. Work in synchronized cell cultures further showed that even though FtsK is recruited early to the septum, dif recombination only occurs shortly before cell division and this activity requires a closing septum. We propose a model whereby septum localization and concentration of FtsK co-ordinate its various roles in chromosome segregation and cell division.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1365-2958
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1018-28
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Delayed activation of Xer recombination at dif by FtsK during septum assembly in Escherichia coli.
pubmed:affiliation
CNRS, Centre de Génétique Moléculaire, UPR 2167, 91198 Gif-sur-Yvette, France; Université Paris-Sud, 91405 Orsay, France; and Université Pierre et Marie Curie- Paris 6, 75005 Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't