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pubmed-article:18343074pubmed:dateCreated2008-6-23lld:pubmed
pubmed-article:18343074pubmed:abstractTextWe evaluated the cost-effectiveness of the restriction enzymes with rare-cutting sites in the genome of Vibrio parahaemolyticus RIMD 2210633 for pulsed-field gel electrophoresis (PFGE) analysis. The evaluation indicated that PFGE with both NotI and SfiI was discriminatory, but NotI was more cost-effective. Based on the results of this study, we suggest using NotI and SfiI as the 1st and the 2nd restriction enzyme for standardizing the PulseNet PFGE protocol for molecular subtyping and global surveillance of V. parahaemolyticus.lld:pubmed
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pubmed-article:18343074pubmed:authorpubmed-author:CamPhung...lld:pubmed
pubmed-article:18343074pubmed:authorpubmed-author:ChiouChien-Sh...lld:pubmed
pubmed-article:18343074pubmed:authorpubmed-author:ChernLih-Ling...lld:pubmed
pubmed-article:18343074pubmed:authorpubmed-author:WangYou-WunYWlld:pubmed
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pubmed-article:18343074pubmed:pagination251-5lld:pubmed
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pubmed-article:18343074pubmed:year2008lld:pubmed
pubmed-article:18343074pubmed:articleTitleEvaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis protocol for rapid subtyping of Vibrio parahaemolyticus.lld:pubmed
pubmed-article:18343074pubmed:affiliationThe Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taichung City 40855, Taiwan.lld:pubmed
pubmed-article:18343074pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:18343074pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:18343074pubmed:publicationTypeEvaluation Studieslld:pubmed