Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-23
pubmed:abstractText
We evaluated the cost-effectiveness of the restriction enzymes with rare-cutting sites in the genome of Vibrio parahaemolyticus RIMD 2210633 for pulsed-field gel electrophoresis (PFGE) analysis. The evaluation indicated that PFGE with both NotI and SfiI was discriminatory, but NotI was more cost-effective. Based on the results of this study, we suggest using NotI and SfiI as the 1st and the 2nd restriction enzyme for standardizing the PulseNet PFGE protocol for molecular subtyping and global surveillance of V. parahaemolyticus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0732-8893
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-5
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Evaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis protocol for rapid subtyping of Vibrio parahaemolyticus.
pubmed:affiliation
The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taichung City 40855, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies