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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-6-13
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pubmed:abstractText |
The dependence of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) flip-flop kinetics on the lateral membrane pressure in a phospholipid bilayer was investigated by sum-frequency vibrational spectroscopy. Planar-supported lipid bilayers were prepared on fused silica supports using the Langmuir-Blodgett/Langmuir-Schaeffer technique, which allows precise control over the lateral surface pressure and packing density of the membrane. The lipid bilayer deposition pressure was varied from 28 to 42 mN/m. The kinetics of lipid flip-flop in these membranes was measured by sum-frequency vibrational spectroscopy at 37 degrees C. An order-of-magnitude difference in the rate constant for lipid translocation (10.9 x 10(-4) s(-1) to 1.03 x 10(-4) s(-1)) was measured for membranes prepared at 28 mN/m and 42 mN/m, respectively. This change in rate results from only a 7.4% change in the packing density of the lipids in the bilayer. From the observed kinetics, the area of activation for native phospholipid flip-flop in a protein-free DPPC planar-supported lipid bilayer was determined to be 73 +/- 12 A(2)/molecule at 37 degrees C. Significance of the observed activation area and potential future applications of the technique to the study of phospholipid flip-flop are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-10969002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-11118525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-11222302,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-12496099,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-1311950,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-1751498,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18339755-9798909
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1542-0086
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
186-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Lateral pressure dependence of the phospholipid transmembrane diffusion rate in planar-supported lipid bilayers.
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pubmed:affiliation |
Department of Chemistry, University of Utah, Salt Lake City, Utah, USA.
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pubmed:publicationType |
Journal Article
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